5'-O-DMTr-2'-OMe-G(iBu)-CE

Product Name: 5'-O-DMTr-2'-OMe-G(iBu)-CE

Product Description:

5'-O-DMTr-2'-OMe-G(iBu)-CE is a high-quality nucleotide derivative specially designed for oligonucleotide synthesis and molecular biology research applications. It is a protected nucleoside phosphoramidite containing a 5'-O-dimethoxytrityl (DMTr) group, a 2'-O-methyl (2'-OMe) modification on the guanine base (G), and an isobutyl (iBu) group. The following features, parameters, and functions highlight the key aspects of this product:

1. Composition: 5'-O-DMTr-2'-OMe-G(iBu)-CE consists of a nucleoside phosphoramidite with a protected guanine base containing a 2'-O-methyl modification. The 5'-O-DMTr group, 2'-OMe modification, and iBu group provide protection during oligonucleotide synthesis.

2. Purity and Quality: 5'-O-DMTr-2'-OMe-G(iBu)-CE is of high purity, ensuring reliable performance and accurate results in oligonucleotide synthesis.

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4. Molecular Modification: The 2'-O-methyl modification on the guanine base offers unique properties and potential biological functions to the synthesized oligonucleotides.

5. Compatibility: 5'-O-DMTr-2'-OMe-G(iBu)-CE is compatible with standard phosphoramidite chemistry and can be used with automated DNA synthesizers.

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Key Features:

1. High Purity: 5'-O-DMTr-2'-OMe-G(iBu)-CE is carefully synthesized and purified to ensure a high level of purity, free from contaminants or impurities.

2. 2'-OMe Modification: The 2'-O-methyl modification on the guanine base imparts stability and alters the base pairing properties of the synthesized oligonucleotides.

3. Multiple Protection Groups: The presence of the DMTr, 2'-OMe modification, and iBu groups provides comprehensive protection during synthesis, ensuring controlled incorporation into the growing oligonucleotide chain.

4. Compatibility: 5'-O-DMTr-2'-OMe-G(iBu)-CE is compatible with standard oligonucleotide synthesis protocols and can be used with automated DNA synthesizers.

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Functions:

1. Oligonucleotide Synthesis: 5'-O-DMTr-2'-OMe-G(iBu)-CE serves as a building block in the automated synthesis of DNA and RNA oligonucleotides. It allows for controlled incorporation of the modified guanine base with a 2'-O-methyl group into the growing oligonucleotide chain.

2. Stability Enhancement: The 2'-O-methyl modification on the guanine base improves the stability of the synthesized oligonucleotides, making them resistant to nuclease degradation.

3. Altered Base Pairing Properties: The 2'-O-methyl modification alters the base pairing properties of the modified guanine, which can be utilized for specific base pairing interactions or for introducing unique functionalities in the oligonucleotide sequence.

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Table: Properties of 5'-O-DMTr-2'-OMe-G(iBu)-CE

In summary, 5'-O-DMTr-2'-OMe-G(iBu)-CE is a high-quality nucleotide phosphoramidite that provides comprehensive protection and a 2'-O-methyl modification to the guanine base during oligonucleotide synthesis. With its high purity, 2'-OMe modification, multiple protection groups, and compatibility with standard protocols, this product is ideal for reliable and controlled synthesis of DNA and RNA oligonucleotides. Researchers can utilize 5'-O-DMTr-2'-OMe-G(iBu)-CE to incorporate the modified guanine base with a 2'-O-methyl group into oligonucleotide sequences, enhancing stability and introducing altered base pairing properties for specific applications in molecular biology research.