5'-O-DMTr-2-F-dG(iBu)-CE 2'-Deoxy-5'-O-DMTr-2'-fluorouridine 3'-CE phosphoramidite

Product Name: 5'-O-DMTr-2-F-dG(iBu)-CE

Product Description:

5'-O-DMTr-2-F-dG(iBu)-CE is a high-quality nucleotide derivative designed for use in oligonucleotide synthesis and molecular biology research applications. It is a protected nucleoside phosphoramidite containing a 5'-O-dimethoxytrityl (DMTr) group, a 2-fluoro (2-F) modification on the deoxyguanosine base (dG), and an isobutyl (iBu) group. The following features, parameters, and functions highlight the key aspects of this product:

1. Composition: 5'-O-DMTr-2-F-dG(iBu)-CE consists of a nucleoside phosphoramidite with a protected 2-fluoro-deoxyguanosine base. The 5'-O-DMTr group, 2-F modification, and iBu group provide protection during oligonucleotide synthesis.

2. Purity and Quality: 5'-O-DMTr-2-F-dG(iBu)-CE is of high purity, ensuring reliable performance and accurate results in oligonucleotide synthesis.

3. Molecular Modification: The 2-fluoro modification on the deoxyguanosine base imparts unique properties and potential biological functions to the synthesized oligonucleotides.

4. Compatibility: 5'-O-DMTr-2-F-dG(iBu)-CE is compatible with standard phosphoramidite chemistry and can be used with automated DNA synthesizers.

Key Features:

1. High Purity: 5'-O-DMTr-2-F-dG(iBu)-CE is carefully synthesized and purified to ensure a high level of purity, free from contaminants or impurities.

2. Fluoro Modification: The 2-fluoro modification on the deoxyguanosine base enhances the stability and alters the base pairing properties of the synthesized oligonucleotides.

3. Multiple Protection Groups: The presence of the DMTr, 2-F modification, and iBu groups provides comprehensive protection during synthesis, allowing for controlled incorporation into the growing oligonucleotide chain.

4. Compatibility: 5'-O-DMTr-2-F-dG(iBu)-CE is compatible with standard oligonucleotide synthesis protocols and can be used with automated DNA synthesizers.

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Functions:

1. Oligonucleotide Synthesis: 5'-O-DMTr-2-F-dG(iBu)-CE serves as a building block in the automated synthesis of DNA and RNA oligonucleotides. It allows for controlled incorporation of the modified 2-fluoro-deoxyguanosine base into the growing oligonucleotide chain.

2. Stability Enhancement: The 2-fluoro modification on the deoxyguanosine base improves the stability of the synthesized oligonucleotides, making them resistant to nuclease degradation.

3. Altered Base Pairing Properties: The 2-fluoro modification alters the base pairing properties of the modified guanosine, which can be utilized for specific base pairing interactions or for introducing unique functionalities in the oligonucleotide sequence.

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Table: Properties of 5'-O-DMTr-2-F-dG(iBu)-CE

In summary, 5'-O-DMTr-2-F-dG(iBu)-CE is a high-quality nucleotide phosphoramidite that provides comprehensive protection and a 2-fluoro modification to the deoxyguanosine base during oligonucleotide synthesis. With its high purity, fluoro modification, multiple protection groups, and compatibility with standard protocols, this product is well-suited for reliable and controlled synthesis of DNA and RNA oligonucleotides. Researchers can utilize 5'-O-DMTr-2-F-dG(iBu)-CE to incorporate the modified 2-fluoro-deoxyguanosine base into oligonucleotide sequences, enhancing stability and introducing altered base pairing properties for specific applications in molecular biology research.