5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE

Product Name: 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE

Product Description:

5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE is a high-quality nucleotide derivative specifically designed for oligonucleotide synthesis and molecular biology research applications. It is a protected nucleoside phosphoramidite containing a 5'-O-dimethoxytrityl (DMTr) group, a 2'-O-tert-butyldimethylsilyl (TBDMS) group, and an isobutyl (iBu) group attached to the guanine base (G). The following features, parameters, and functions highlight the key aspects of this product:

Composition: 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE consists of a nucleoside phosphoramidite with a protected guanine base. The 5'-O-DMTr group, 2'-O-TBDMS group, and iBu group provide protection during oligonucleotide synthesis.

Purity and Quality: 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE is of high purity, ensuring reliable performance and accurate results in oligonucleotide synthesis.

Molecular Protection: The DMTr, TBDMS, and iBu groups protect the nucleotide during synthesis, preventing unwanted reactions and ensuring controlled incorporation into the growing oligonucleotide chain.

Compatibility: 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE is compatible with standard phosphoramidite chemistry and can be used with automated DNA synthesizers.

Key Features:

High Purity: 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE is carefully synthesized and purified to ensure a high level of purity, free from contaminants or impurities.

Multiple Protection Groups: The presence of the DMTr, TBDMS, and iBu groups provides comprehensive protection to the nucleotide during synthesis, allowing for controlled and selective deprotection steps.

Compatibility: 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE is compatible with standard oligonucleotide synthesis protocols and can be used with automated DNA synthesizers.

Functions:

Oligonucleotide Synthesis: 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE is used as a building block in the automated synthesis of DNA and RNA oligonucleotides. It allows for controlled incorporation of the protected guanine base into the growing oligonucleotide chain.

Solid-Phase Synthesis: The protection groups on 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE enable efficient solid-phase synthesis of oligonucleotides, ensuring high yields and purity of the final product.

Functional Modification: The protected guanine base can be further modified or used as a starting point for introducing additional functional groups or labels into the oligonucleotide sequence.

In summary, 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE is a high-quality nucleotide phosphoramidite that provides comprehensive protection to the guanine base during oligonucleotide synthesis. With its high purity, multiple protection groups, and compatibility with standard protocols, this product is well-suited for reliable and controlled synthesis of DNA and RNA oligonucleotides. Researchers can utilize 5'-O-DMTr-2'-O-TBDMS-G(iBu)-CE in solid-phase synthesis to incorporate the protected guanine base into oligonucleotide sequences, allowing for further functional modification or labeling.