Dimer Phosphoramidites-Towards Higher Yields and Purity Control for Oligonucleotide APIs

• Introduction

Allbiosyn’s team of scientists constantly strives to work on novel and innovative solutions to tackle difficult problems in RNA therapeutics development such as scale-up and manufacturing challenges. The following section highlights some examples of Allbiosyn’s creative product solutions to address limitations of current solid phase oligonucleotide synthesis and enhance the capabilities of existing systems and platforms.

• Dimer Phosphoramidites------Towards Higher Yields and Purity Control for Oligonucleotide APIs

Synthetic Advantages

1) Convergent building blocks for siRNA and ASO-----Reduced SPPS Steps, Elevated Overall Yields

2) Lower Solvent Consumption---Greener Alternative

3) Lower Production Cost

4)Fewer byproducts and better synthetic control

All 16 2'-OMe dimer phosphoramidites are available individually or as a set. Dimers consisting of 2'-F, 2'-OMOE modified units are available upon custom request.

• 5'-Phosphoramidites, an alternative to special 5'-nucleoside-solid support and UnyLinker-solid support

Benefits of 5’-Phosphoramidites

1)Automated synthesis compatible

2)Choice of solid support based on pore size, particle size, swelling property, and other desirable properties

3)Choice of inexpensive solid support to save production cost

4)Improved yield of the first coupling reaction

• 5'-Labelled Phosphoramidites